Curriculum Vitae

Centro de Ciências e Tecnologias Nucleares (C2TN)


Name: Paula Dolores
Surnames: Galhofas Raposinho
Born: 28-07-1964, Damaia, Amadora, Portugal
Nationality: Portuguese

Phone: +351 21 994 6225/6242/6241
Fax: +351 21 994 6185
E-mail: paular (@ctn.ist.utl.pt)


Education

  • 2002 – PhD in Sciences (Biochemistry), Faculty of Sciences, University of Geneva, Switzerland.
  • 1988 – Graduation in Biochemistry, Faculty of Sciences, University of Lisbon.

Professional Employment Career

  • Since 2003 – Researcher, at the Inorganic and Radiopharmaceutical Chemistry Group, Department of Chemistry, Instituto Tecnológico e Nuclear (ITN).
  • 1997-2003 – Research Assistant (PhD student), Division of Pediatric Endocrinology and Diabetology, University of Medicine, Geneva, Switzerland.
  • 1994-1997 – Research Assistant, Instituto Tecnológico e Nuclear (ITN).
  • 1990-1994 – Research Trainee, Department of Radioisotopes, (ITN, former INETI).

Fellowships and Training

  • 1986-1990 – INETI/ICEN fellowship, at Department of Radioisotopes of Instituto Ciência e Energia Nuclear (ICEN) with Doctor Isabel Rego dos Santos.
  • 1992 (November) - IAEA fellowship at the Netria Laboratory, St. Bartholomew's Hospital, London, "Radioimmunoassay Methodologies".
  • 1994-1995 – Fellowship of Confederation of Grants for foreign Students in Switzerland, Division of Pediatric Endocrinology and Diabetology, Cantonal Hospital, Geneva, Switzerland, with Prof. M. L. Aubert’s, "The effect of chronic administration of ethanol on the reproductive function in the male adult rat and in the rat during sexual development".
  • 1996 – "Nuclear Receptors: Molecular Biology and Genetics of the Retinoic Signaling Pathway", Instituto Gulbenkian de Oeiras.
  • 1998 – 2nd Postgraduate Course in Molecular and Cellular Endocrinology, European Federation of Endocrine Societes (EFES), Turku, Finland.
  • 2000 – "Modèles transgéniques en recherche biomédicale: principes, gestion des projets et aspects pratiques", University of Geneva/Charles River-Iffa Credo (with the collaboration of the Organization Veterinary Cantonal of Genebra, OVCG), Geneva, Switzerland.
  • 2002 – "Education for Persons Responsible for Directing Animals Experiments (LTK Module 2)", Institut für Labortierkunde, University of Zurich, Switzerland.

Main Scientific Areas of Research

  • Radioimmunoassay (1986-1988).
  • Neuroendocrinology and in vivo pharmacology of neuropeptides and their analogues (1997-2003).
  • Radiopharmaceuticals development (after 2003).

Teaching activities

  • Collaboration in the training of Radioimmunoassay (Technical Nuclear Medicine Course students, ESTeST-Lisbon, "Nuclear Medicine" specialized doctors of Oncology Portuguese Institute and of Santa Maria Hospital of Lisbon, Biochemistry students of the University of Lisbon)-1988-1997
  • Collaboration in the training of Radiopharmacy ("Nuclear Medicine" specialized doctors of Santa Cruz Hospital)
  • Collaboration in the course "Cours d'Expérimentation Animale", CHUV, Lausanne, Switzerland.

Participation in Projects

  • Radiolabeling of L-Arginine Analogues with the Unit fac-[99mTc(CO)3]+ for Probing Nitric Oxide Synthase (NOS) in vivo: Synthesis, Characterization and Biological Evaluation, POCI/SAU-FCF/58855/2004.

  • Novel Radioactive Probes for Molecular Imaging of Sigma Receptors, POCI/QUI/59588/2004.

Scientific Works

  • Graduation thesis "Desenvolvimento de um método de doseamento do cortisol, por radioimunoensaio", Faculty of Sciences of University of Lisbon (1988).
  • Trainee to research assistant thesis in ITN "Doseamento Radioimunológico de Hormonas Tiroideias: Métodos de Separação", Sacavém, Portugal (1988).
  • Monograph "Receptores Hormonais de Membrana: Transdução do Sinal", ITN, Sacavém, Portugal (1988).
  • PhD Thesis "Potential role in the neuroendocrine regulation of growth and reproduction of various peptides that are primarily involved in the hypothalamic control of feeding and energy balance ", University of Geneva, Switzerland (2002).
  • 7 original publications as first author in refereed journals.
  • 18 communications to national and international meetings (5 oral presentations).

Selected Publications

  • Can, Daniel; Spingler, Bernhard; Raposinho, Paula; et al.; (Cp-R)M(CO)3 (M=Re or 99mTc) Arylsulfonamide, Arylsulfamide, and Arylsulfamate Conjugates for Selective Targeting of Human Carbonic Anhydrase IX, Angewandte Chemie-Internat. Ed, 2012, 51, 3354-3357.
    Journal impact factor: 13.734, Citations: 12.
  • Raposinho, Paula D.; Correia, Joao D. G.; Oliveira, Maria Cristina; Santos, Isabel; Melanocortin-1 Receptor-Targeting With Radiolabeled Cyclic alpha-Melanocyte-Stimulating Hormone Analogs for Melanoma Imaging, Biopolymers, 2010, 94,820-829.
    Journal impact factor:  2.572, Citations: 10.
  • Oliveira, Bruno L.; Correia, Joao D. G.; Raposinho, Paula D.; Santos, Isabel; Ferreira, Antonio et.al., Re and Tc-99m organometallic complexes containing pendant L-arginine derivatives as potential probes of inducible nitric oxide synthase, Dalton Transactions, 2009, 152-162.
    Journal impact factor:  4.081, Citations: 12.
  • Raposinho, Paula; Xavier, Catarina; Falcao, Soraia; et.al., Melanoma targeting with alpha-melanocyte stimulating hormone analogs labeled with Tc-99m(CO)(3) (+): effect of cyclization on tumor-seeking properties, J. Biological Inorganic Chemistry, 2008,13,449-459.
    Journal impact factor:  3.600, Citations: 28.
  • Alves S, Vitor R, Raposinho PD, Marques F, Correia JDG, Paulo A, Santos I, Metal-based drugs for diagnosis and therapy,  In Metal Ions in Biologie and Medicine, Vol. 9, Eds: Alpoim MC, Morais PV, Santos MA, Cristovão AJ, Centeno JA, Collery P, John Libbey Eurotext, Paris, 2006 pp 3-8.
  • Raposinho PD, Pedrazzini T, White RB, Palmiter RD, Aubert ML, Chronic neuropeptide Y infusion into the lateral ventricle induces sustained feeding and obesity in mice lacking either Npy1r or Npy5r expression,  Endocrinology, 2004, 145 (1): 304-310.
  • Raposinho PD, White RB, Aubert ML, The Melanocortin agonist Melanotan-II (MT-II) reduces the orexigenic and adipogenic effects of Neuropeptide Y (NPY) but does not affect the NPY-driven suppressive effects on the gonadotropic and somatotropic axes in the male rat,  J of Neuroendocrinology, 2003, 15: 173-181.
  • Raposinho PD, Pierroz DD, Broqua P, White RB, Pedrazzini T, Aubert ML, Chronic administration of Neuropeptide Y into the lateral ventricle of C57BL/6J male mice produces an obesity syndrome including hyperphagia, hyperleptonemia, insulin resistance, and hypogonadism,  Molecular Cellular Endocrinology, 2001, 185: 195-204.
  • Raposinho PD, Castillo E, d’Allèves V, Broqua P, Pralong PF, Aubert ML, Chronic Blockade of the Melanocortin 4 Receptor Subtype leads to Obesity independently of Neuropeptide Y action, with no adverse effects on the Gonadotropic and Somatotropic Axes,  Endocrinology, 2000, 141: 4419-4427.
  • Raposinho PD, Broqua P, Hayward A, Akinsanya K, Galyean R, Schteingart C, Junien JL, Aubert ML, Stimulation of the Gonadotropic Axis by the Neuropeptide Y Receptor Y1 Antagonist/Y4 Agonist 1229U91 in the male Rat,  Neuroendocrinology, 2000, 71: 2-7, (Rapid communication).
  • Raposinho PD, Broqua P, Pierroz DD, Hayward A, Dumont Y, Quirion R, Junien JL, Aubert ML, Evidence that the inhibition of Luteinizing Hormone secretion exerted by central administration of Neuropeptide Y (NPY) in the rat is predominantly mediated by the NPY-Y5 Receptor Subtype,  Endocrinology, 1999, 140: 4046-4055.