Curriculum Vitae

Centro de Ciências e Tecnologias Nucleares (C2TN)


Photo.

Name: Filipa Fernandes
Surname: Mendes
Born: Lisboa, Portugal, 1st October 1976
Nationality: Portuguese

Phone: +351 21 994 6196
Fax: +351 21 994 6016
E-mail: fmendes (add @ctn.ist.utl.pt)
DeGóis Platform. see curriculum vitae


Education

  • 2000-2004 – PhD in Cellular Biology, Faculty of Sciences, University of Lisbon. Final classification: summa cum laude.
  • 1994-1999 – BSc in Biology, Faculty of Sciences, University of Lisbon.
    Final classification: 18 (over 20).

Scientific Activities and Present Position

  • Sept 2013 – Principal Researcher, FCT Investigator Development Grant (Life and Health Sciences), Radiopharma-ceutical Sciences Group, Centro de Ciências e Tecnologias Nucleares (C2TN), Instituto Superior Técnico, Universidade de Lisboa, Campus Tecnológico e Nuclear.
  • Sept 2008/Aug 2013 – Auxiliary Researcher (Ciência 2007) in the Radiopharmaceutical Sciences Group, Chemical and Radiopharmaceutical Sciences Unit, ITN;
  • Jan 2005/Jul 2008 – Post-doctoral Fellow at Center of Human Genetics, National Institute of Health Dr. Ricardo Jorge (Lisboa) and Faculty of Sciences, University of Lisboa under the supervision of Prof. Margarida D Amaral.
  • May/Aug 2003 – Visiting Graduate Research Fellow at the Department of Biology, University of Virginia, USA under the supervision of Prof. Margarida Barroso.
  • 2000-2004 – Graduate Research Fellow (PhD candidate) at the Laboratory of Molecular Biology of the Center of Human Genetics, National Institute of Health Dr. Ricardo Jorge (Lisbon) under the supervision of Dr Deborah Penque.
  • Set 1998/Jul 1999 – Undergraduate trainee (Senior Thesis), at the Center of Human Genetics, National Institute of Health Dr. Ricardo Jorge (Lisbon) under the supervision of Dr Deborah Penque and Prof Rui Malhó.

Fellowships and Training

  • 2010/May 2014 “Inhibition of PARP-1 by metal-based anticancer compounds”, Short Term Scientific Mission to the Laboratory of Organometallic and Medicinal Chemistry, EPFL, Lausanne, Switzerland, supervised by Profs. Paul Dyson and Angela Casini.
  • Apr/May 2007 – "High Throughput Microscopy for Systems Biology", EMB0 Practical Course, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
  • Jun/Jul 2005 – "Role of novel proteins in CFTR trafficking" - 4 weeks of training at the Department of Cell Biology, EMBL, Heidelberg, Germany, supervised by Dr Rainer Pepperkok.
  • 13/June 2005 – "Microinjection and Detection of Probes in Cells", EMBL Practical Course, EMBL Heidelberg, Germany.
  • 2005/2007 – Post-Doctoral fellowship PRAXIS XXI/BPD/04 from the Fundação para a Ciência e a Tecnologia (Foundation for Science and Technology), Portugal.
  • Aug 2003 – "Detection of CFTR by in vitro phosphorylation and cell surface biotinylation" – 2 weeks of training at the Department of Cell Biology and Gregory Fleming James Cystic Fibrosis Research Center, University of Alabama at Birmingham, USA, supervised by Prof. Zsuzsa Bebok.
  • Set/Oct 2000 – "Measurement of Chloride Fluxes by MQAE" – 4 weeks of training at the Department of Medical Cell Biology, Uppsala University, Uppsala, Sweden, supervised by Prof. Godfried Roomans.
  • Mar/Apr 2000 – "Intracelular localization of normal and mutant CFTR protein: techniques of thawed frozen section colloidal gold immunocytochemistry" - 4 weeks of training at the Sir William Dunn School of Pathology, Oxford, UK, supervised by Prof. David J Vaux.
  • 2000/2003 – PhD fellowship PRAXIS XXI/BD/21440/99, from the Foundation for Science and Technology (FCT), Portugal.
  • 1999 – PRODEP/99 (EU) fellowship for undergraduate training.

Main Scientific Areas of Research

  • Molecular imaging and radiopharmaceutical sciences.
  • Biology of Human ABC Transporter Diseases.

Scientific Specialization

  • Biology and Health Sciences - Molecular and Cellular Biology.

Present Research Interests

Focus on merging advances in molecular biology with chemistry (radiopharmaceutical and medicinal):

  • Study of new probes for in vivo molecular imaging by SPECT and PET and/or targeted radiotherapy: Identification of new targets and tudy of the biological mechanisms and molecular interactions;
  • Elucidation of the mechanism of action of novel anticancer metal complexes (gold, platinum and ruthenium based) – particularly inhibition of zinc metalloenzymes (PARP-1);
  • Study of the interactions of metal-based anticancer compounds with biomolecules - particular attention to proteins as possible targets for the pharmacological and toxicological activity.

Teaching Activities

  • Since Sept 2013 – Invited Assistant Professor, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa (average: 3h/week).
  • April 2008/July 2012 – Invited Assistant Professor, Pharmacogenomics, MSc Pharmacy, Universidade Lusófona de Humanidades e Tecnologia. (5 semesters ~ 6h /week).

Membership of Professional Societies

  • Member of the Cystic Fibrosis European Network.
  • Member of the American Society of Cell Biology.
  • Member of Sociedade Portuguesa de Microscopia Electrónica e Biologia Celular.
  • Member of Ordem dos Biólogos.

Awards

  • 2006 – "Medalha de Honra L’Oréal Portugal para as Mulheres na Ciência" (L’Oreal Award for Women in Science), L’Oréal Portugal, Unesco and Fundação para a Ciência e a Tecnologia, Portugal.
  • 2005 – "Prémio Gulbenkian de Estímulo à Investigação" (Gulbenkian Award for Research Incentive) Calouste Gulbenkian Foundation, Portugal.

Scientific Works

Theses:

  • PhD Thesis: "Expression, Localization and Traffic of Wild-Type and Mutant CFTR Protein".
    BSc Thesis: "Cystic fibrosis: Expression and Intracellular localization of CFTR protein".

Other works:

  • 30 original publications in international refereed journals (Researcher ID:B-2057-2010 h index:10).
  • 12 published abstracts.
  • 11 oral communications in national and international meetings (7 invited).
  • 35 poster communications in national and international meetings.
  • 2 patents.

Participation in Projects

  • 2013 EXPL/BIM-MEC/0115/2012 (FCT): "A Molecular Imaging Approach to Cystic Fibrosis", Principal Researcher.
  • 2013 EXCL/QEQ-MED/0233/2012 (FCT): "Molecular and Nano Tools for Cancer Theranostics".
  • 2012-2015 COST Action CM1105 (EU): "Functional metal complexes that bind to biomolecules".
  • 2012-2014 Acções Integradas Luso-Espanholas.
  • 2012 E-23/12 Bilateral Actions Spain-Portugal: "Target-specific and Heterobimetallic Platinum Complexes: Synthesis, Characterization and Mechanistic Studies".
  • 2012-2015 PTDC/QUI-QUI/121752/2010 (FCT): "Nitric Oxide Synthase targeting with Re(I)/99mTc(I)-complexes containing L-Arg derivatives: A structure-activity study", team member.
  • 2012-2015 PTDC/QUI-QUI/118077/2010 (FCT): "Preclinical evaluation of ruthenium potential drugs for cancer therapy", team member.
  • 2011-2013 PTDC/QUI-QUI/114139/2009 (FCT): "Targeting telomerase inhibition with new anti-tumoral Cu(II) Complexes", team member
  • 2009-2012 PTDC/QUI-QUI/102049/2008 (FCT): "Radiolabeled Benzazole Derivatives for In Vivo Imaging of Amyloid Aggregation", team member.
  • 2009-2010 COST D39 (EU): "Metallo-drug design and action",  team member.

Selected Publications

  • Can D, Spingler B, Schmutz P, Mendes F, Raposinho P, Fernandes C, Carta F, Innocenti A, Santos I, Supuran C, Alberto R (2012) “[(Cp-R)M(CO)3] (M = Re or 99mTc) Sulphonamide Conjugates for Selective Targeting of Human Carbonic Anhydrase IX” Angewandte Chem, 51:3354–3357.
    Journal impact factor: 13,460, Citations: : 11.
  • Mendes F, Groessl M, Nazarov AA, Tsybin YO, Sava G, Santos I, Dyson PJ, Casini A (2011) “Metal-Based Inhibition of Poly(ADP-ribose) Polymerase – The Guardian Angel of DNA” J Med Chemistry, 54:2196–2206.
    Journal impact factor: 5,248, Citations: 30.
  • Mendes F, Paulo A, Santos I (2011) “Metalloprobes for functional monitoring of tumour multidrug resistance by nuclear imaging” Review, Dalton Trans.,40:5377-5393.
    Journal impact factor: 3,838, Citations: 3.
  • Esteves T, Gama S, Mendes F, Marques F, Paulo A, Pessoa J, Rino J, Santos I (2010) “Tricarbonyl M (I) (M = Re, 99mTc) Complexes Bearing Acridine Fluorophores: Synthesis, Characterization, DNA Interaction Studies and Nuclear Targeting” Org Biomol Chem, 8:4104–4116.
    Journal impact factor: 3,451, Citations: 9.

Other Publications

  • Mendes F, Farinha CM, Felício V, Alves PC, Vieira I, Amaral MD (2012) "BAG-1 Stabilizes Mutant F508del-CFTR in an Ubiquitin-Like-Domain-Dependent Manner", Cell Physiol Biochem, 30:1120-33.
  • Li H, Yang W, Mendes F, Amaral MD, Sheppard D (2012), "Impact of the cystic fibrosis mutation F508del-CFTR on renal cyst formation and growth", Am J Physiol - Renal Physiol, 303:F1176-86.
  • Tomaz AI, Jakusch T, Morais TS, Marques F, Almeida RFM, Mendes F, Enyedy EA, Santos I, Pessoa JC, Kiss T, Garcia MH (2012), "[RuII(h5-C5H5)(bipy)(PPh3)]+, a promising large spectrum antitumor agent: cytotoxic activity and interaction with Human Serum Albumin", J Inorg Bioch, 117:261-69.
  • Moura C, Gano L, Mendes F, Raposinho PD, Abrantes AM, Botelho MF, Santos I, Paulo A (2012), "99mTc(I)/Re(I) Tricarbonyl Complexes for In Vivo Targeting of Melanotic Melanoma: Synthesis and Biological Evaluation", Eur J Med Chem, 50:350-360.
  • Mendes F, Gano L, Fernandes C, Paulo A, Santos I (2012), "Studies of the myocardial uptake and excretion mechanisms of a novel 99mTc heart perfusion agent", Nucl Med Biol, 39:207–213.
  • Francisco C, Gama S, Mendes F, Marques F, Santos IC, Santos I, Paulo A (2011), "Pt(II) Complexes with Bidentate and Tridentate Pyrazolyl-containing Chelators: Synthesis, Structural Characterization and Biological Studies", Dalton Trans., 40:5781-5792.
  • Gama S, Mendes F, Marques F, Santos IC, Correia I, Costa Pessoa J, Santos I, Paulo A (2011), "Copper(II) complexes anchored by tridentate pyrazol-based ligands: Synthesis, characterization, DNA cleavage activity and cytotoxicity", J Inorg Bioch, 105:637–644
  • Oliveira BL, Raposinho P, Mendes F, Figueira F, Santos I, Ferreira A, Cordeiro C, Freire AP, Correia JDG (2010), "Re and Tc Tricarbonyl Complexes: From the Suppression of NO Biosynthesis in Macrophages to in vivo Targeting of Inducible Nitric Oxide Synthase", Bioconj Chem, 21:2168–2172